حضور گونه‌های Phytophthora در آب‌های جاری کشاورزی در استان فارس

The dissemination of Phytophthora spp. in the surface irrigation water was studied during 1993-94 in Marvdasht area and certain other parts of Fars province. Monthly the water samples were taken along 100Km of the Kor river 22Km up stream from the Doroudzan Dam to about 80Km down stream to Mardvdasht where Sivand and Kor rivers joined. The other sampling locations includede the Maeen river adjecent to the Dam, and the Sivand river from Dashte-Morghab to Mardasht. Few samples were also obtained from Shapour and Dalaky rivers and also from Yasouj and the Sisakht regions in Khkilueh and Boir Ahmad. The water temperature, pH and EC were measured and samples were assayed on the same day of the collection. The water sample was filtered through the two layers of the cheese cloth and about 1L was placed in a plastic container (30 × 20 Cm) 2-3C deep and 100 fresh citrus leaf disks (6mm dia) were also added and incubated at the room temprature. After 48hr, disks were collected, washed, blotted dry and plated on Phytophthora were collected, washed, blotted dry and plated on Phytophthora selective medium (PARPH). At least 50 disks were used in the 5 plates for each sample. The five to 8 boiled hemp seeds were placed on each growing colony and transferred to the distilled water, after 12hr for sporangium production. The Phytophthora species were idenified by their morphological characteristic and temperature requirement. Phytophthora spp. were present in the most rivers except the Shapour and Dalaky. The predominant species were non-popilate high temperature (above 35C) species mainly P. cryptogea and P. drechsleri. In some cases P. capcisi and the other unidentified species were also detected. Phytophthora spp. were present throughout the year but the lowest population was detected during winter months. The fluctuation of the phytophthora population and the other water microorganisms in the Kor river after shiraz petrochemical complex during the growing season was very high, but sometimes reduced to zero (perhaps due to the water pollution). No Phytophthora colonies were detected in the dam lake or in the most cases in the cement canals.